Sperm nuclear instability and staining with aniline blue: abnormal persistance of histones in spermatozoa in infertile men
نویسندگان
چکیده
منابع مشابه
Evaluation of Protamine Level in Human Sperm Samples Using Chromomycin A3 and Aniline Blue Staining
Background: Current microscopic experimental methods cannot diagnose DNA damages present in spermatozoa .Therefore, some methods are needed to address the abnormality of the genetic material status on the sperm samples. As reported by many investigators aniline blue staining technique has been used for identifying sperm chromatin condensation. Also, chromomycin A3 is used for evaluation of the ...
متن کاملRelationship between Sperm Parameters with Sperm Function Tests in Infertile Men with at Least One Failed Cycle after Intracytoplasmic Sperm Injection Cycle
Objective Imbalance between production of reactive oxygen species (ROS) and total antioxidant capacity in testis, epididymis, and seminal fluid can eventually lead to subfertility and infertility. Abnormal chromatin packaging, and DNA fragmentation is considered as the main underlying causes. In this regard, we aimed to assess relationship between sperm parameters with DNA damage, protamine def...
متن کاملevaluation of protamine level in human sperm samples using chromomycin a3 and aniline blue staining
background: current microscopic experimental methods cannot diagnose dna damages present in spermatozoa .therefore, some methods are needed to address the abnormality of the genetic material status on the sperm samples. as reported by many investigators aniline blue staining technique has been used for identifying sperm chromatin condensation. also, chromomycin a3 is used for evaluation of the ...
متن کاملThe utility of sperm DNA damage assay using toluidine blue and aniline blue staining in routine semen analysis
OBJECTIVE The aim of the present study was to examine the relationship among male age, strict morphology, and sperm chromatin structure and condensation. METHODS Sperm samples from a total of 100 men underwent semen analysis, and sperm chromatin structure and condensation were assessed with toluidine blue (TB) and aniline blue (AB) tests. RESULTS Prevalence of strict morphology of less than...
متن کاملAbnormal meiotic recombination in infertile men and its association with sperm aneuploidy.
Defects in early meiotic events are thought to play a critical role in male infertility; however, little is known regarding the relationship between early meiotic events and the chromosomal constitution of human sperm. Thus, we analyzed testicular tissue from 26 men (9 fertile and 17 infertile men), using immunofluorescent techniques to examine meiotic chromosomes, and fluorescent in situ hybri...
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ژورنال
عنوان ژورنال: International Journal of Andrology
سال: 1992
ISSN: 0105-6263,1365-2605
DOI: 10.1111/j.1365-2605.1992.tb01132.x